PrimePCR™ SYBR® Green Assay: SSX6, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0054069

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: SSX6, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: SSX6, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0046808
Assay Design:   exonic
Chromosome Location:   X:47979515-47979627question
Amplicon Length:   83
Splice Variants Targeted:   ENST00000298181 ENST00000336777 ENST00000337502 ENST00000375515 ENST00000276049 ENST00000376932 ENST00000319275 ENST00000376923 ENST00000311798 ENST00000347757 ENST00000376919 ENST00000376909 ENST00000298396 ENST00000376895 ENST00000376886 ENST00000376884 ENST00000396928 ENST00000594102 ENST00000601554 ENST00000594778 ENST00000594016 ENST00000596327 ENST00000596574 ENST00000600668 ENST00000596036 ENST00000595320 ENST00000595689 ENST00000595235 ENST00000598733 ENST00000593457 ENST00000593835 ENST

Gene Information

Description Not Available

Gene Symbol:   SSX6
Gene Name:   synovial sarcoma, X breakpoint 6 (pseudogene)
Aliases:   SSXP2, dJ54B20.1, psiSSX2
RefSeq:   NC_000023.10 NT_079573.4
Ensembl:   ENSG00000171483
Entrez:   280657
Chromosome Mapping:   Xp11.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.996900
y-intercept 36.640000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download