This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCEP0053893
PrimePCR™ PreAmp for SYBR® Green Assay: KCNK9, Human
PrimePCR™ Template for SYBR® Green Assay: KCNK9, Human
This gene encodes a member of the superfamily of potassium channel proteins containing two pore-forming P domains and is highly expressed in the cerebellum. Amplification and overexpression of this gene has been observed in several types of human carcinomas notably in breast cancer. This gene is imprinted in fetal brain with preferential expression from the maternal allele. [provided by RefSeq Oct 2010]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.