PrimePCR™ SYBR® Green Assay: TRIM49, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0053851

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: TRIM49, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: TRIM49, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0046590
Assay Design:   exonic
Chromosome Location:   11:89531525-89531664question
Amplicon Length:   110
Splice Variants Targeted:   ENST00000417545 ENST00000449290 ENST00000244891 ENST00000329758 ENST00000532501 ENST00000448984 ENST00000332682

Gene Information

The protein encoded by this gene contains a RING zinc finger a motif known to be involved in protein-protein interactions. This gene has been found to be preferentially expressed in testis. Related pseudogenes and gene duplicates have also been identified on chromosome 11. [provided by RefSeq Aug 2010]

Gene Symbol:   TRIM49
Gene Name:   tripartite motif containing 49
Aliases:   RNF18, TRIM49L2
RefSeq:   NC_000011.9 NT_167190.1
Ensembl:   ENSG00000168930
Entrez:   57093
Chromosome Mapping:   11p11.12-q12

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.995800
y-intercept 35.790000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download