PrimePCR™ SYBR® Green Assay: STIP1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0053813

List Price:    $138.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0046552
Assay Design:   exonic
Chromosome Location:   11:63971076-63971608question
Amplicon Length:   72
Splice Variants Targeted:   ENST00000358794 ENST00000305218 ENST00000538945 ENST00000540887

Gene Information

STIP1 is an adaptor protein that coordinates the functions of HSP70 (see HSPA1A; MIM 140550) and HSP90 (see HSP90AA1; MIM 140571) in protein folding. It is thought to assist in the transfer of proteins from HSP70 to HSP90 by binding both HSP90 and substrate-bound HSP70. STIP1 also stimulates the ATPase activity of HSP70 and inhibits the ATPase activity of HSP90 suggesting that it regulates both the conformations and ATPase cycles of these chaperones (Song and Masison 2005 [PubMed 16100115]).[supplied by OMIM Jul 2009]

Gene Symbol:   STIP1
Gene Name:   stress-induced-phosphoprotein 1
Aliases:   HOP, IEF-SSP-3521, P60, STI1, STI1L
RefSeq:   NC_000011.9 NG_016360.1 NT_167190.1
Ensembl:   ENSG00000168439
Entrez:   10963
UniGene:   Hs.337295
Chromosome Mapping:   11q13

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 36.880000
Efficiency 93

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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