PrimePCR™ SYBR® Green Assay: SERPINF2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0053745

List Price:    $138.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0046484
Assay Design:   exonic
Chromosome Location:   17:1650677-1650801question
Amplicon Length:   95
Splice Variants Targeted:   ENST00000453066 ENST00000450523 ENST00000382061 ENST00000324015

Gene Information

This gene encodes a member of the serpin family of serine protease inhibitors. The protein is a major inhibitor of plasmin which degrades fibrin and various other proteins. Consequently the proper function of this gene has a major role in regulating the blood clotting pathway. Mutations in this gene result in alpha-2-plasmin inhibitor deficiency which is characterized by severe hemorrhagic diathesis. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Sep 2009]

Gene Symbol:   SERPINF2
Gene Name:   serpin peptidase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 2
Aliases:   A2AP, AAP, ALPHA-2-PI, API, PLI
RefSeq:   NC_000017.10 NG_013215.1 NT_010718.16
Ensembl:   ENSG00000167711
Entrez:   5345
UniGene:   Hs.159509
Chromosome Mapping:   17p13

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998500
y-intercept 36.240000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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