This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCEP0053616
PrimePCR™ PreAmp for SYBR® Green Assay: FBN1, Human
PrimePCR™ Template for SYBR® Green Assay: FBN1, Human
This gene encodes a member of the fibrillin family. The encoded protein is a large extracellular matrix glycoprotein that serve as a structural component of 10-12 nm calcium-binding microfibrils. These microfibrils provide force bearing structural support in elastic and nonelastic connective tissue throughout the body. Mutations in this gene are associated with Marfan syndrome isolated ectopia lentis autosomal dominant Weill-Marchesani syndrome MASS syndrome and Shprintzen-Goldberg craniosynostosis syndrome. [provided by RefSeq Jul 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.