PrimePCR™ SYBR® Green Assay: VWA2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0053591

List Price:    $138.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0046330
Assay Design:   exonic
Chromosome Location:   10:116049108-116049243question
Amplicon Length:   106
Splice Variants Targeted:   ENST00000298715 ENST00000392982

Gene Information

Description Not Available

Gene Symbol:   VWA2
Gene Name:   von Willebrand factor A domain containing 2
Aliases:   AMACO, CCSP-2, MGC157755, NET42
RefSeq:   NC_000010.10 NT_030059.13
Ensembl:   ENSG00000165816
Entrez:   340706
UniGene:   Hs.735561
Chromosome Mapping:   10q25.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998800
y-intercept 35.970000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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