PrimePCR™ SYBR® Green Assay: ARMC3, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0053556

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: ARMC3, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: ARMC3, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0046295
Assay Design:   exonic
Chromosome Location:   10:23235181-23247999question
Amplicon Length:   107
Splice Variants Targeted:   ENST00000298032 ENST00000409983 ENST00000409049 ENST00000447081 ENST00000376528

Gene Information

Armadillo/beta-catenin (CTNNB1; MIM 116806)-like (ARM) domains are imperfect 45-amino acid repeats involved in protein-protein interactions. ARM domain-containing proteins such as ARMC3 function in signal transduction development cell adhesion and mobility and tumor initiation and metastasis (Li et al. 2006 [PubMed 16915934]).[supplied by OMIM Mar 2008]

Gene Symbol:   ARMC3
Gene Name:   armadillo repeat containing 3
Aliases:   CT81, FLJ25845, FLJ32827, KU-CT-1
RefSeq:   NC_000010.10 NT_008705.16
Ensembl:   ENSG00000165309
Entrez:   219681
Chromosome Mapping:   10p12.31

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.997000
y-intercept 35.920000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download