PrimePCR™ SYBR® Green Assay: RBM47, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0053381

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: RBM47, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: RBM47, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0046120
Assay Design:   exonic
Chromosome Location:   4:40440850-40440940question
Amplicon Length:   61
Splice Variants Targeted:   ENST00000379308 ENST00000381793 ENST00000381795 ENST00000295971 ENST00000515053 ENST00000513473 ENST00000505414 ENST00000514782 ENST00000507180 ENST00000511598 ENST00000511902 ENST00000505220 ENST00000319592 ENST00000377939 ENST00000377948 ENST00000286298 ENST00000361820 ENST00000368378 ENST00000457183 ENST00000541990 ENST00000541576

Gene Information

Description Not Available

Gene Symbol:   RBM47
Gene Name:   RNA binding motif protein 47
Aliases:   DKFZp686F02235, FLJ20273, FLJ21344, FLJ21643, NET18
RefSeq:   NC_000004.11 NT_006238.11
Ensembl:   ENSG00000163694
Entrez:   54502
Chromosome Mapping:   4p14

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998400
y-intercept 36.850000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download