PrimePCR™ SYBR® Green Assay: MNDA, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0053363

List Price:    $174.00
Your Price:   Log In
Quantity:  
 
Add to Custom Plate

PrimePCR™ PreAmp for SYBR® Green Assay: MNDA, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: MNDA, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0046102
Assay Design:   exonic
Chromosome Location:   1:158815691-158817532question
Amplicon Length:   88
Splice Variants Targeted:   ENST00000368141 ENST00000438394

Gene Information

The myeloid cell nuclear differentiation antigen (MNDA) is detected only in nuclei of cells of the granulocyte-monocyte lineage. A 200-amino acid region of human MNDA is strikingly similar to a region in the proteins encoded by a family of interferon-inducible mouse genes designated Ifi-201 Ifi-202 and Ifi-203 that are not regulated in a cell- or tissue-specific fashion. The 1.8-kb MNDA mRNA which contains an interferon-stimulated response element in the 5-prime untranslated region was significantly upregulated in human monocytes exposed to interferon alpha. MNDA is located within 2200 kb of FCER1A APCS CRP and SPTA1. In its pattern of expression and/or regulation MNDA resembles IFI16 suggesting that these genes participate in blood cell-specific responses to interferons. [provided by RefSeq Jul 2008]

Gene Symbol:   MNDA
Gene Name:   myeloid cell nuclear differentiation antigen
Aliases:   PYHIN3
RefSeq:   NC_000001.10 NT_004487.19
Ensembl:   ENSG00000163563
Entrez:   4332
Chromosome Mapping:   1q22

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999100
y-intercept 36.170000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download