PrimePCR™ SYBR® Green Assay: STT3B, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0053357

List Price:    $138.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0046096
Assay Design:   exonic
Chromosome Location:   3:31621313-31621453question
Amplicon Length:   111
Splice Variants Targeted:   ENST00000295770

Gene Information

The SIMP protein contains a highly immunogenic minor histocompatibility antigen epitope of 9 amino acids B6(dom1). Like ITM1 (MIM 601134) SIMP is homologous to yeast STT3 an oligosaccharyltransferase essential for cell proliferation (McBride et al. 2002 [PubMed 12439619]).[supplied by OMIM Mar 2008]

Gene Symbol:   STT3B
Gene Name:   STT3, subunit of the oligosaccharyltransferase complex, homolog B (S. cerevisiae)
Aliases:   FLJ90106, SIMP, STT3-B
RefSeq:   NC_000003.11 NT_022517.18
Ensembl:   ENSG00000163527
Entrez:   201595
UniGene:   Hs.475812
Chromosome Mapping:   3p23

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.997500
y-intercept 36.080000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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