PrimePCR™ SYBR® Green Assay: HDAC11, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0053354

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: HDAC11, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: HDAC11, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0046093
Assay Design:   exonic
Chromosome Location:   3:13521844-13522853question
Amplicon Length:   84
Splice Variants Targeted:   ENST00000433119 ENST00000295757 ENST00000402259 ENST00000402271 ENST00000404548 ENST00000404040 ENST00000458642 ENST00000405478 ENST00000405025 ENST00000522202 ENST00000418189 ENST00000434848 ENST00000416248 ENST00000455904 ENST00000437379 ENST00000446613 ENST00000263967

Gene Information

This gene encodes a class IV histone deacetylase. The encoded protein is localized to the nucleus and may be involved in regulating the expression of interleukin 10. Alternative splicing results in multiple transcript variants.[provided by RefSeq Apr 2009]

Gene Symbol:   HDAC11
Gene Name:   histone deacetylase 11
Aliases:   FLJ22237, HD11
RefSeq:   NC_000003.11 NT_022517.18
Ensembl:   ENSG00000163517
Entrez:   79885
Chromosome Mapping:   3p25.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998900
y-intercept 36.710000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download