PrimePCR™ SYBR® Green Assay: RNF25, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0053346

List Price:    $146.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0046085
Assay Design:   exonic
Chromosome Location:   2:219528589-219528719question
Amplicon Length:   101
Splice Variants Targeted:   ENST00000295704

Gene Information

The protein encoded by this gene contains a RING finger motif. The mouse counterpart of this protein has been shown to interact with Rela the p65 subunit of NF-kappaB (NFKB) and modulate NFKB-mediated transcription activity. The mouse protein also binds ubiquitin-conjugating enzymes (E2s) and is a substrate for E2-dependent ubiquitination. [provided by RefSeq Jul 2008]

Gene Symbol:   RNF25
Gene Name:   ring finger protein 25
Aliases:   AO7, FLJ13906
RefSeq:   NC_000002.11 NG_008018.1 NT_005403.17
Ensembl:   ENSG00000163481
Entrez:   64320
UniGene:   Hs.471403
Chromosome Mapping:   2q35

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 37.680000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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