PrimePCR™ SYBR® Green Assay: SLC15A2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best efficiency; Same primer pair as used in probe assay qHsaCEP0053338

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: SLC15A2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: SLC15A2, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0046077
Assay Design:   exonic
Chromosome Location:   3:121631875-121634093question
Amplicon Length:   83
Splice Variants Targeted:   ENST00000489711 ENST00000295605 ENST00000469013

Gene Information

The mammalian kidney expresses a proton-coupled peptide transporter that is responsible for the absorption of small peptides as well as beta-lactam antibiotics and other peptide-like drugs from the tubular filtrate. This transporter SLC15A2 belongs to the same gene family as SLC15A1 (MIM 600544) the proton-coupled peptide transporter found in the small intestine (Liu et al 1995 [PubMed 7756356]).[supplied by OMIM Feb 2011]

Gene Symbol:   SLC15A2
Gene Name:   solute carrier family 15 (H+/peptide transporter), member 2
Aliases:   FLJ33407, PEPT2
RefSeq:   NC_000003.11 NT_005612.16
Ensembl:   ENSG00000163406
Entrez:   6565
Chromosome Mapping:   3q13.33

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998900
y-intercept 36.290000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download