PrimePCR™ SYBR® Green Assay: APOA1BP, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0053334

List Price:    $174.00
Your Price:   Log In
Quantity:  
 
Add to Custom Plate

PrimePCR™ PreAmp for SYBR® Green Assay: APOA1BP, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: APOA1BP, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0046073
Assay Design:   exonic
Chromosome Location:   1:156561558-156561760question
Amplicon Length:   173
Splice Variants Targeted:   ENST00000596251 ENST00000595337 ENST00000336034 ENST00000335624 ENST00000344120 ENST00000264659 ENST00000578925 ENST00000542707 ENST00000584266 ENST00000297873 ENST00000369774 ENST00000355946 ENST00000342085 ENST00000441549 ENST00000389224 ENST00000354836 ENST00000343932 ENST00000379540 ENST00000379521 ENST00000307365 ENST00000574788 ENST00000250066 ENST00000332776 ENST00000304328 ENST00000530342 ENST00000308783 ENST00000536867 ENST00000360620 ENST00000358173 ENST00000360351 ENST00000447185 ENST

Gene Information

The product of this gene interacts with apolipoprotein A-I (apoA-I) the major apolipoprotein of high-density lipoproteins (HDLs). It is secreted into some bodily fluids and its synthesis and secretion are stimulated in vitro by incubating cells with apoA-I. The human genome contains related pseudogenes. [provided by RefSeq Jul 2008]

Gene Symbol:   APOA1BP
Gene Name:   apolipoprotein A-I binding protein
Aliases:   AIBP, MGC119143, MGC119144, MGC119145, YJEFN1
RefSeq:   NC_000001.10 NT_004487.19
Ensembl:   ENSG00000163382
Entrez:   128240
Chromosome Mapping:   1q21

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999100
y-intercept 36.630000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download