PrimePCR™ SYBR® Green Assay: IVL, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0053315

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: IVL, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: IVL, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0046054
Assay Design:   exonic
Chromosome Location:   1:152884247-152884361question
Amplicon Length:   85
Splice Variants Targeted:   ENST00000368764 ENST00000392667

Gene Information

Involucrin a component of the keratinocyte crosslinked envelope is found in the cytoplasm and crosslinked to membrane proteins by transglutaminase. This gene is mapped to 1q21 among calpactin I light chain trichohyalin profillaggrin loricrin and calcyclin. [provided by RefSeq Jul 2008]

Gene Symbol:   IVL
Gene Name:   involucrin
Aliases:   Not Available
RefSeq:   NC_000001.10 NT_004487.19
Ensembl:   ENSG00000163207
Entrez:   3713
Chromosome Mapping:   1q21

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999000
y-intercept 36.110000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download