PrimePCR™ SYBR® Green Assay: PEX19, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0053267

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: PEX19, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: PEX19, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0046006
Assay Design:   exonic
Chromosome Location:   1:160249279-160249374question
Amplicon Length:   66
Splice Variants Targeted:   ENST00000368072 ENST00000495624 ENST00000440949 ENST00000492597 ENST00000370325

Gene Information

This gene is necessary for early peroxisomal biogenesis. It acts both as a cytosolic chaperone and as an import receptor for peroxisomal membrane proteins (PMPs). Peroxins (PEXs) are proteins that are essential for the assembly of functional peroxisomes. The peroxisome biogenesis disorders (PBDs) are a group of genetically heterogeneous autosomal recessive lethal diseases characterized by multiple defects in peroxisome function. These disorders have at least 14 complementation groups with more than one phenotype being observed for some complementation groups. Although the clinical features of PBD patients vary cells from all PBD patients exhibit a defect in the import of one or more classes of peroxisomal matrix proteins into the organelle. Defects in this gene are a cause of Zellweger syndrome (ZWS) as well as peroxisome biogenesis disorder complementation group 14 (PBD-CG14) which is also known as PBD-CGJ. Alternative splicing results in multiple transcript variants. [provided by RefSeq Aug 2010]

Gene Symbol:   PEX19
Gene Name:   peroxisomal biogenesis factor 19
Aliases:   D1S2223E, FLJ55296, HK33, PMP1, PMPI, PXF, PXMP1
RefSeq:   NC_000001.10 NT_004487.19 NG_008637.1
Ensembl:   ENSG00000162735
Entrez:   5824
Chromosome Mapping:   1q23.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.996700
y-intercept 36.180000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download