PrimePCR™ SYBR® Green Assay: HENMT1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0053262

List Price:    $138.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0046001
Assay Design:   exonic
Chromosome Location:   1:109193654-109193803question
Amplicon Length:   120
Splice Variants Targeted:   ENST00000370031 ENST00000370032 ENST00000420055 ENST00000402983

Gene Information

Description Not Available

Gene Symbol:   HENMT1
Gene Name:   HEN1 methyltransferase homolog 1 (Arabidopsis)
Aliases:   C1orf59, FLJ30525, HEN1, MGC111091
RefSeq:   NC_000001.10 NT_032977.9
Ensembl:   ENSG00000162639
Entrez:   113802
UniGene:   Hs.7962
Chromosome Mapping:   1p13.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.995500
y-intercept 35.840000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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