PrimePCR™ SYBR® Green Assay: DHRS3, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0053246

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: DHRS3, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: DHRS3, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0045985
Assay Design:   exonic
Chromosome Location:   1:12640592-12677164question
Amplicon Length:   79
Splice Variants Targeted:   ENST00000376223 ENST00000430996

Gene Information

Short-chain dehydrogenases/reductases (SDRs) such as DHRS3 catalyze the oxidation/reduction of a wide range of substrates including retinoids and steroids (Haeseleer and Palczewski 2000 [PubMed 10800688]).[supplied by OMIM Jun 2009]

Gene Symbol:   DHRS3
Gene Name:   dehydrogenase/reductase (SDR family) member 3
Aliases:   RDH17, Rsdr1, SDR1, SDR16C1, retSDR1
RefSeq:   NC_000001.10 NT_021937.19
Ensembl:   ENSG00000162496
Entrez:   9249
Chromosome Mapping:   1p36.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999100
y-intercept 36.170000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download