PrimePCR™ SYBR® Green Assay: LY6K, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0053181

List Price:    $138.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0045920
Assay Design:   exonic
Chromosome Location:   8:143782040-143784517question
Amplicon Length:   102
Splice Variants Targeted:   ENST00000570580 ENST00000574968 ENST00000571924 ENST00000572300 ENST00000292430 ENST00000518841 ENST00000519387 ENST00000522591 ENST00000561179

Gene Information

Description Not Available

Gene Symbol:   LY6K
Gene Name:   lymphocyte antigen 6 complex, locus K
Aliases:   CT97, FLJ35226, HSJ001348, ly-6K
RefSeq:   NC_000008.10 NT_008046.16
Ensembl:   ENSG00000160886
Entrez:   54742
UniGene:   Hs.69517
Chromosome Mapping:   8q24.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998100
y-intercept 35.610000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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