PrimePCR™ SYBR® Green Assay: TMEM190, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0053160

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: TMEM190, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: TMEM190, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0045899
Assay Design:   exonic
Chromosome Location:   19:55889384-55889530question
Amplicon Length:   117
Splice Variants Targeted:   ENST00000320736 ENST00000234420 ENST00000340249 ENST00000397661 ENST00000592199 ENST00000587760 ENST00000585575 ENST00000588228 ENST00000587260 ENST00000341650 ENST00000338779 ENST00000597002 ENST00000436070 ENST00000291934 ENST00000425217 ENST00000367321 ENST00000367308 ENST00000441122 ENST00000423867 ENST00000367307 ENST00000450802 ENST00000376592 ENST00000376590 ENST00000376585 ENST00000376583 ENST00000355083 ENST00000296440 ENST00000358536

Gene Information

Description Not Available

Gene Symbol:   TMEM190
Gene Name:   transmembrane protein 190
Aliases:   MDAC1, MGC149301, MGC149302
RefSeq:   NC_000019.9 NT_011109.16
Ensembl:   ENSG00000160472
Entrez:   147744
Chromosome Mapping:   19q13.42

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.996400
y-intercept 35.970000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download