PrimePCR™ SYBR® Green Assay: TOR2A, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0053157

List Price:    $142.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0045896
Assay Design:   exonic
Chromosome Location:   9:130493806-130493930question
Amplicon Length:   95
Splice Variants Targeted:   ENST00000336067 ENST00000373284 ENST00000373281 ENST00000458505

Gene Information

This gene encodes a member of the AAA family of adenosine triphosphatases with similarity to Clp proteases and heat shock proteins. Alternative splicing at this locus results in the translation of multiple isoforms of the encoded protein some of which contain salusin peptides in the C-terminal region. These peptides may play roles in hypotension myocardial growth and the induction of mitogenesis and may also be involved in the pathogenesis of atherosclerosis. [provided by RefSeq Oct 2011]

Gene Symbol:   TOR2A
Gene Name:   torsin family 2, member A
Aliases:   FLJ14771, MGC99558, TORP1
RefSeq:   NC_000009.11 NT_008470.19
Ensembl:   ENSG00000160404
Entrez:   27433
UniGene:   Hs.444106
Chromosome Mapping:   9q34.11

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 36.480000
Efficiency 94

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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