PrimePCR™ SYBR® Green Assay: LRRC36, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0053126

List Price:    $146.00
Your Price:   Log In
 
Add to Custom Plate

Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0045865
Assay Design:   exonic
Chromosome Location:   16:67381485-67397507question
Amplicon Length:   84
Splice Variants Targeted:   ENST00000569499 ENST00000329956 ENST00000568804 ENST00000563189 ENST00000435835 ENST00000290940 ENST00000541146

Gene Information

Description Not Available

Gene Symbol:   LRRC36
Gene Name:   leucine rich repeat containing 36
Aliases:   FLJ11004, RORBP70, XLHSRF2
RefSeq:   NC_000016.9 NT_010498.15
Ensembl:   ENSG00000159708
Entrez:   55282
UniGene:   Hs.125139
Chromosome Mapping:   16q22.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 36.330000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download