PrimePCR™ SYBR® Green Assay: RGL4, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0053120

List Price:    $146.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0045859
Assay Design:   exonic
Chromosome Location:   22:24041076-24041185question
Amplicon Length:   80
Splice Variants Targeted:   ENST00000300648 ENST00000262518 ENST00000546530 ENST00000295101 ENST00000544049 ENST00000553525 ENST00000555571 ENST00000553530 ENST00000556437 ENST00000355512 ENST00000404301 ENST00000406236 ENST00000407322 ENST00000434263 ENST00000402788 ENST00000343854 ENST00000302956 ENST00000380913 ENST00000418909 ENST00000588649 ENST00000215591 ENST00000342740 ENST00000334420 ENST00000265626 ENST00000218104 ENST00000527990 ENST00000254579 ENST00000358077 ENST00000472284 ENST00000408954 ENST00000491893 ENST

Gene Information

Description Not Available

Gene Symbol:   RGL4
Gene Name:   ral guanine nucleotide dissociation stimulator-like 4
Aliases:   MGC119678, MGC119680, Rgr
RefSeq:   NC_000022.10 NT_011520.12
Ensembl:   ENSG00000159496
Entrez:   266747
UniGene:   Hs.658997
Chromosome Mapping:   22q11.23

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998700
y-intercept 35.840000
Efficiency 103

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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