PrimePCR™ SYBR® Green Assay: PLA2G4D, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0053113

List Price:    $174.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0045852
Assay Design:   exonic
Chromosome Location:   15:42360073-42360195question
Amplicon Length:   93
Splice Variants Targeted:   ENST00000290472

Gene Information

The phospholipase A2 enzyme family including PLA2G4D catalyze the hydrolysis of glycerophospholipids at the sn-2 position and then liberate free fatty acids and lysophospholipids (Chiba et al. 2004 [PubMed 14709560]).[supplied by OMIM Jun 2009]

Gene Symbol:   PLA2G4D
Gene Name:   phospholipase A2, group IVD (cytosolic)
Aliases:   cPLA2delta
RefSeq:   NC_000015.9 NT_010194.17
Ensembl:   ENSG00000159337
Entrez:   283748
Chromosome Mapping:   15q15.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999100
y-intercept 36.130000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download