PrimePCR™ SYBR® Green Assay: EPB41, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0053097

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: EPB41, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: EPB41, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0045836
Assay Design:   exonic
Chromosome Location:   1:29313964-29314106question
Amplicon Length:   113
Splice Variants Targeted:   ENST00000373800 ENST00000349460 ENST00000347529 ENST00000373798 ENST00000373797 ENST00000343067 ENST00000356093 ENST00000398863

Gene Information

The protein encoded by this gene together with spectrin and actin constitute the red cell membrane cytoskeletal network. This complex plays a critical role in erythrocyte shape and deformability. Mutations in this gene are associated with type 1 elliptocytosis (EL1). Alternatively spliced transcript variants encoding different isoforms have been described for this gene.[provided by RefSeq Oct 2009]

Gene Symbol:   EPB41
Gene Name:   erythrocyte membrane protein band 4.1 (elliptocytosis 1, RH-linked)
Aliases:   4.1R, EL1, HE
RefSeq:   NC_000001.10 NT_004610.19 NG_013344.1
Ensembl:   ENSG00000159023
Entrez:   2035
Chromosome Mapping:   1p33-p32

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 36.660000
Efficiency 94

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download