This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCEP0053093
PrimePCR™ PreAmp for SYBR® Green Assay: DEDD, Human
PrimePCR™ Template for SYBR® Green Assay: DEDD, Human
This gene encodes a protein that contains a death effector domain (DED). DED is a protein-protein interaction domain shared by adaptors regulators and executors of the programmed cell death pathway. Overexpression of this gene was shown to induce weak apoptosis. Upon stimulation this protein was found to translocate from cytoplasm to nucleus and colocalize with UBTF a basal factor required for RNA polymerase I transcription in the nucleolus. At least three transcript variants encoding the same protein have been found for this gene. [provided by RefSeq Jul 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.