This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCEP0053090
PrimePCR™ PreAmp for SYBR® Green Assay: F11R, Human
PrimePCR™ Template for SYBR® Green Assay: F11R, Human
Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. The protein encoded by this immunoglobulin superfamily gene member is an important regulator of tight junction assembly in epithelia. In addition the encoded protein can act as (1) a receptor for reovirus (2) a ligand for the integrin LFA1 involved in leukocyte transmigration and (3) a platelet receptor. Multiple 5' alternatively spliced variants encoding the same protein have been identified but their biological validity has not been established. [provided by RefSeq Jul 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.