PrimePCR™ SYBR® Green Assay: GDPD5, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0053082

List Price:    $146.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0045821
Assay Design:   exonic
Chromosome Location:   11:75146602-75147983question
Amplicon Length:   72
Splice Variants Targeted:   ENST00000526177 ENST00000533784 ENST00000529721 ENST00000336898 ENST00000533805 ENST00000534322 ENST00000376282 ENST00000443276

Gene Information

Glycerophosphodiester phosphodiesterases (GDPDs; EC 3.1.4.46) such as GDPD5 are involved in glycerol metabolism (Lang et al. 2008 [PubMed 17578682]).[supplied by OMIM Jan 2010]

Gene Symbol:   GDPD5
Gene Name:   glycerophosphodiester phosphodiesterase domain containing 5
Aliases:   GDE2, PP1665
RefSeq:   NC_000011.9 NT_167190.1
Ensembl:   ENSG00000158555
Entrez:   81544
UniGene:   Hs.503297
Chromosome Mapping:   11q13.4-q13.5

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 36.060000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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