PrimePCR™ SYBR® Green Assay: SKI, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0053055

List Price:    $142.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0045794
Assay Design:   exonic
Chromosome Location:   1:2238289-2238427question
Amplicon Length:   109
Splice Variants Targeted:   ENST00000378536

Gene Information

This gene encodes the nuclear protooncogene protein homolog of avian sarcoma viral (v-ski) oncogene. It functions as a repressor of TGF-beta signaling and may play a role in neural tube development and muscle differentiation. [provided by RefSeq Oct 2009]

Gene Symbol:   SKI
Gene Name:   v-ski sarcoma viral oncogene homolog (avian)
Aliases:   SKV
RefSeq:   NC_000001.10 NG_013084.1 NT_004350.19
Ensembl:   ENSG00000157933
Entrez:   6497
UniGene:   Hs.656507
Chromosome Mapping:   1q22-q24

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999100
y-intercept 36.380000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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