PrimePCR™ SYBR® Green Assay: MEGF11, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0053051

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: MEGF11, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: MEGF11, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0045790
Assay Design:   exonic
Chromosome Location:   15:66220864-66222190question
Amplicon Length:   105
Splice Variants Targeted:   ENST00000571199 ENST00000576903 ENST00000574557 ENST00000371521 ENST00000344894 ENST00000427047 ENST00000458322 ENST00000570827 ENST00000285199 ENST00000570475 ENST00000302956 ENST00000382350 ENST00000341547 ENST00000357063 ENST00000409699 ENST00000288745 ENST00000455812 ENST00000422354 ENST00000395625 ENST00000395614 ENST00000360698 ENST00000307635 ENST00000394329 ENST00000335110 ENST00000369697 ENST00000537408

Gene Information

Description Not Available

Gene Symbol:   MEGF11
Gene Name:   multiple EGF-like-domains 11
Aliases:   DKFZp434L121, KIAA1781
RefSeq:   NC_000015.9 NT_010194.17 NW_003315944.1
Ensembl:   ENSG00000157890
Entrez:   84465
Chromosome Mapping:   15q22.31

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 36.190000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download