PrimePCR™ SYBR® Green Assay: SLC37A3, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0053049

List Price:    $138.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0045788
Assay Design:   exonic
Chromosome Location:   7:140035106-140035212question
Amplicon Length:   77
Splice Variants Targeted:   ENST00000340308 ENST00000447932 ENST00000326232 ENST00000469636 ENST00000498469 ENST00000491357 ENST00000485538 ENST00000492027 ENST00000477006

Gene Information

Description Not Available

Gene Symbol:   SLC37A3
Gene Name:   solute carrier family 37 (glycerol-3-phosphate transporter), member 3
Aliases:   FLJ16367, MGC32939
RefSeq:   NC_000007.13 NT_007914.15
Ensembl:   ENSG00000157800
Entrez:   84255
UniGene:   Hs.446021
Chromosome Mapping:   7q34

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998300
y-intercept 36.240000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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