PrimePCR™ SYBR® Green Assay: ZNF19, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0053033

List Price:    $138.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0045772
Assay Design:   exonic
Chromosome Location:   16:71518502-71518614question
Amplicon Length:   83
Splice Variants Targeted:   ENST00000288177 ENST00000565637 ENST00000564230 ENST00000568815 ENST00000564225 ENST00000566202 ENST00000569072

Gene Information

The protein encoded by this gene contains a zinc finger a nucleic acid-binding domain present in many transcription factors. This gene is located in a region next to ZNF23 a gene also encoding a zinc finger protein on chromosome 16. [provided by RefSeq Jul 2008]

Gene Symbol:   ZNF19
Gene Name:   zinc finger protein 19
Aliases:   KOX12, MGC51021
RefSeq:   NC_000016.9 NT_010498.15
Ensembl:   ENSG00000157429
Entrez:   7567
UniGene:   Not Available
Chromosome Mapping:   16q22

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 35.700000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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