PrimePCR™ SYBR® Green Assay: ARSE, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0053032

List Price:    $142.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0045771
Assay Design:   exonic
Chromosome Location:   X:2871226-2873466question
Amplicon Length:   61
Splice Variants Targeted:   ENST00000381134 ENST00000438544 ENST00000540563 ENST00000545496

Gene Information

Arylsulfatase E is a member of the sulfatase family. It is glycosylated postranslationally and localized to the golgi apparatus. Sulfatases are essential for the correct composition of bone and cartilage matrix. X-linked chondrodysplasia punctata a disease characterized by abnormalities in cartilage and bone development has been linked to mutations in this gene. [provided by RefSeq Jul 2008]

Gene Symbol:   ARSE
Gene Name:   arylsulfatase E (chondrodysplasia punctata 1)
Aliases:   CDPX, CDPX1, CDPXR, MGC163310
RefSeq:   NC_000023.10 NG_007091.1 NT_167197.1
Ensembl:   ENSG00000157399
Entrez:   415
UniGene:   Hs.386975
Chromosome Mapping:   Xp22.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 36.790000
Efficiency 94

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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