PrimePCR™ SYBR® Green Assay: ANKRD9, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0052984

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: ANKRD9, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: ANKRD9, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0045723
Assay Design:   exonic
Chromosome Location:   14:102974035-102974124question
Amplicon Length:   60
Splice Variants Targeted:   ENST00000576782 ENST00000572970 ENST00000575445 ENST00000570958 ENST00000371521 ENST00000344894 ENST00000427047 ENST00000458322 ENST00000565208 ENST00000393701 ENST00000568461 ENST00000288040 ENST00000449317 ENST00000404125 ENST00000559651 ENST00000286918 ENST00000560748 ENST00000559404 ENST00000557902 ENST00000309664 ENST00000379709 ENST00000395144 ENST00000266031 ENST00000320295 ENST00000395143 ENST00000388913 ENST00000261937 ENST00000393347 ENST00000502649 ENST00000590071 ENST00000270288

Gene Information

Description Not Available

Gene Symbol:   ANKRD9
Gene Name:   ankyrin repeat domain 9
Aliases:   MGC21990
RefSeq:   NC_000014.8 NT_026437.12
Ensembl:   ENSG00000156381
Entrez:   122416
Chromosome Mapping:   14q32.31

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 36.750000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download