PrimePCR™ SYBR® Green Assay: VPS37A, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0052963

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: VPS37A, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: VPS37A, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0045702
Assay Design:   exonic
Chromosome Location:   8:17104758-17104874question
Amplicon Length:   87
Splice Variants Targeted:   ENST00000333244 ENST00000602312 ENST00000526559 ENST00000164133 ENST00000532850 ENST00000527441 ENST00000267068 ENST00000392346 ENST00000419322 ENST00000435881 ENST00000246912 ENST00000346833 ENST00000591024 ENST00000530878 ENST00000361897 ENST00000258930 ENST00000539011 ENST00000557846 ENST00000314100 ENST00000419706 ENST00000268057 ENST00000395205 ENST00000268482 ENST00000324849 ENST00000518038 ENST00000521829 ENST00000521005 ENST00000324815 ENST00000216181 ENST00000255008 ENST00000553310 ENST

Gene Information

Description Not Available

Gene Symbol:   VPS37A
Gene Name:   vacuolar protein sorting 37 homolog A (S. cerevisiae)
Aliases:   FLJ32642, FLJ42616, HCRP1, PQBP2
RefSeq:   NC_000008.10 NT_167187.1
Ensembl:   ENSG00000155975
Entrez:   137492
Chromosome Mapping:   8p22

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998700
y-intercept 36.150000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download