PrimePCR™ SYBR® Green Assay: JMJD5, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0052948

List Price:    $142.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0045687
Assay Design:   exonic
Chromosome Location:   16:27221560-27221688question
Amplicon Length:   99
Splice Variants Targeted:   ENST00000380948 ENST00000568965 ENST00000562733 ENST00000441782 ENST00000569329 ENST00000286096

Gene Information

Description Not Available

Gene Symbol:   JMJD5
Gene Name:   jumonji domain containing 5
Aliases:   FLJ13798
RefSeq:   NC_000016.9 NT_010393.16
Ensembl:   ENSG00000155666
Entrez:   79831
UniGene:   Hs.145717
Chromosome Mapping:   16p12.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998700
y-intercept 36.620000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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