This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCEP0052935
PrimePCR™ PreAmp for SYBR® Green Assay: USP25, Human
PrimePCR™ Template for SYBR® Green Assay: USP25, Human
Ubiquitin (MIM 191339) is a highly conserved 76-amino acid protein involved in regulation of intracellular protein breakdown cell cycle regulation and stress response. Ubiquitin is released from degraded proteins by disassembly of the polyubiquitin chains which is mediated by ubiquitin-specific proteases (USPs) such as USP25 (Valero et al. 1999 [PubMed 10644437]).[supplied by OMIM Mar 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.