PrimePCR™ SYBR® Green Assay: PPP4R1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0052917

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: PPP4R1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: PPP4R1, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0045656
Assay Design:   exonic
Chromosome Location:   18:9588230-9588845question
Amplicon Length:   111
Splice Variants Targeted:   ENST00000247306 ENST00000369585 ENST00000419847 ENST00000451932 ENST00000443182 ENST00000552116 ENST00000415373 ENST00000444402 ENST00000415345 ENST00000550393 ENST00000375976 ENST00000211379 ENST00000404765 ENST00000375964 ENST00000257974 ENST00000413922 ENST00000454478 ENST00000412012 ENST00000546462 ENST00000422756 ENST00000425649 ENST00000434446 ENST00000552467 ENST00000400556 ENST00000400555 ENST00000584074 ENST00000581250 ENST00000580745 ENST00000582240 ENST00000581835 ENST00000449450 ENST

Gene Information

Description Not Available

Gene Symbol:   PPP4R1
Gene Name:   protein phosphatase 4, regulatory subunit 1
Aliases:   PP4(Rmeg), PP4R1
RefSeq:   NC_000018.9 NT_010859.14
Ensembl:   ENSG00000154845
Entrez:   9989
Chromosome Mapping:   18p11.22

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 37.660000
Efficiency 94

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download