PrimePCR™ SYBR® Green Assay: SH3RF1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0052901

List Price:    $142.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0045640
Assay Design:   exonic
Chromosome Location:   4:170190306-170190448question
Amplicon Length:   113
Splice Variants Targeted:   ENST00000284637 ENST00000502315 ENST00000510806

Gene Information

This gene encodes a protein containing an N-terminus RING-finger four SH3 domains and a region implicated in binding of the Rho GTPase Rac. Via the RING-finger the encoded protein has been shown to function as an ubiquitin-protein ligase involved in protein sorting at the trans-Golgi network. The encoded protein may also act as a scaffold for the c-Jun N-terminal kinase signaling pathway facilitating the formation of a functional signaling module. [provided by RefSeq Jul 2008]

Gene Symbol:   SH3RF1
Gene Name:   SH3 domain containing ring finger 1
Aliases:   FLJ21602, KIAA1494, POSH, RNF142, SH3MD2
RefSeq:   NC_000004.11 NT_016354.19
Ensembl:   ENSG00000154447
Entrez:   57630
UniGene:   Hs.301804
Chromosome Mapping:   4q32.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 36.170000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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