This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCEP0052901
PrimePCR™ PreAmp for SYBR® Green Assay: SH3RF1, Human
PrimePCR™ Template for SYBR® Green Assay: SH3RF1, Human
This gene encodes a protein containing an N-terminus RING-finger four SH3 domains and a region implicated in binding of the Rho GTPase Rac. Via the RING-finger the encoded protein has been shown to function as an ubiquitin-protein ligase involved in protein sorting at the trans-Golgi network. The encoded protein may also act as a scaffold for the c-Jun N-terminal kinase signaling pathway facilitating the formation of a functional signaling module. [provided by RefSeq Jul 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.