PrimePCR™ SYBR® Green Assay: OBSCN, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0052896

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: OBSCN, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: OBSCN, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0045635
Assay Design:   exonic
Chromosome Location:   1:228471352-228471441question
Amplicon Length:   60
Splice Variants Targeted:   ENST00000343304 ENST00000284548 ENST00000570156 ENST00000366706 ENST00000483539 ENST00000422127 ENST00000359599 ENST00000366707 ENST00000366709

Gene Information

The obscurin gene spans more than 150 kb contains over 80 exons and encodes a protein of approximately 720 kDa. The encoded protein contains 68 Ig domains 2 fibronectin domains 1 calcium/calmodulin-binding domain 1 RhoGEF domain with an associated PH domain and 2 serine-threonine kinase domains. This protein belongs to the family of giant sacromeric signaling proteins that includes titin and nebulin and may have a role in the organization of myofibrils during assembly and may mediate interactions between the sarcoplasmic reticulum and myofibrils. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq Jul 2008]

Gene Symbol:   OBSCN
Gene Name:   obscurin, cytoskeletal calmodulin and titin-interacting RhoGEF
Aliases:   ARHGEF30, DKFZp666E245, FLJ14124, KIAA1556, KIAA1639, MGC120409, MGC120410, MGC120411, MGC120412, MGC138590, UNC89
RefSeq:   NC_000001.10 NT_167186.1
Ensembl:   ENSG00000154358
Entrez:   84033
Chromosome Mapping:   1q42.13

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998900
y-intercept 35.860000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download