PrimePCR™ SYBR® Green Assay: JAZF1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0052872

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: JAZF1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: JAZF1, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0045611
Assay Design:   exonic
Chromosome Location:   7:27880352-27880448question
Amplicon Length:   67
Splice Variants Targeted:   ENST00000589359 ENST00000534797 ENST00000297273 ENST00000441802 ENST00000511217 ENST00000397683 ENST00000397682 ENST00000397679 ENST00000401884 ENST00000405547 ENST00000310397 ENST00000342631 ENST00000427814 ENST00000283928 ENST00000430432 ENST00000447620 ENST00000382303 ENST00000382297 ENST00000382293 ENST00000265018 ENST00000595360 ENST00000264033 ENST00000428004 ENST00000564777 ENST00000316900 ENST00000561656 ENST00000316911 ENST00000300843

Gene Information

This gene encodes a nuclear protein with three C2H2-type zinc fingers and functions as a transcriptional repressor. Chromosomal aberrations involving this gene are associated with endometrial stromal tumors. Alternatively spliced variants which encode different protein isoforms have been described; however not all variants have been fully characterized [provided by RefSeq Jul 2008]

Gene Symbol:   JAZF1
Gene Name:   JAZF zinc finger 1
Aliases:   DKFZp761K2222, TIP27, ZNF802
RefSeq:   NC_000007.13 NG_011499.1 NT_007819.17
Ensembl:   ENSG00000153814
Entrez:   221895
Chromosome Mapping:   7p15.2-p15.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998200
y-intercept 35.760000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download