PrimePCR™ SYBR® Green Assay: RMND5A, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0052866

List Price:    $142.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0045605
Assay Design:   exonic
Chromosome Location:   2:87001168-87001312question
Amplicon Length:   115
Splice Variants Targeted:   ENST00000283632

Gene Information

Description Not Available

Gene Symbol:   RMND5A
Gene Name:   required for meiotic nuclear division 5 homolog A (S. cerevisiae)
Aliases:   CTLH, FLJ12753, FLJ13910, FLJ21795, MGC78451, RMD5, p44CTLH
RefSeq:   NC_000002.11 NT_022184.15
Ensembl:   ENSG00000153561
Entrez:   64795
UniGene:   Hs.75277
Chromosome Mapping:   2p11.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 35.610000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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