This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCEP0052859
PrimePCR™ PreAmp for SYBR® Green Assay: RBMS1, Human
PrimePCR™ Template for SYBR® Green Assay: RBMS1, Human
This gene encodes a member of a small family of proteins which bind single stranded DNA/RNA. These proteins are characterized by the presence of two sets of ribonucleoprotein consensus sequence (RNP-CS) that contain conserved motifs RNP1 and RNP2 originally described in RNA binding proteins and required for DNA binding. These proteins have been implicated in such diverse functions as DNA replication gene transcription cell cycle progression and apoptosis. Several transcript variants resulting from alternative splicing and encoding different isoforms have been described. A pseudogene for this locus is found on chromosome 12. [provided by RefSeq Feb 2009]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.