PrimePCR™ SYBR® Green Assay: CAPSL, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0052835

List Price:    $142.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0045574
Assay Design:   exonic
Chromosome Location:   5:35910046-35910474question
Amplicon Length:   109
Splice Variants Targeted:   ENST00000397367 ENST00000513623 ENST00000514524 ENST00000397366

Gene Information

Description Not Available

Gene Symbol:   CAPSL
Gene Name:   calcyphosine-like
Aliases:   MGC26610
RefSeq:   NC_000005.9 NT_006576.16
Ensembl:   ENSG00000152611
Entrez:   133690
UniGene:   Hs.55150
Chromosome Mapping:   5p13.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998700
y-intercept 36.140000
Efficiency 94

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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