PrimePCR™ SYBR® Green Assay: MEPE, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0052834

List Price:    $146.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0045573
Assay Design:   exonic
Chromosome Location:   4:88766202-88766314question
Amplicon Length:   83
Splice Variants Targeted:   ENST00000395102 ENST00000497649 ENST00000511670 ENST00000361056 ENST00000424957 ENST00000560249 ENST00000540395

Gene Information

Description Not Available

Gene Symbol:   MEPE
Gene Name:   matrix extracellular phosphoglycoprotein
Aliases:   OF45
RefSeq:   NC_000004.11 NT_016354.19
Ensembl:   ENSG00000152595
Entrez:   56955
UniGene:   Hs.676357
Chromosome Mapping:   4q21.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999100
y-intercept 36.170000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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