This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0052821
PrimePCR™ PreAmp for SYBR® Green Assay: TGOLN2, Human
PrimePCR™ Template for SYBR® Green Assay: TGOLN2, Human
This gene encodes a type I integral membrane protein that is localized to the trans-Golgi network a major sorting station for secretory and membrane proteins. The encoded protein cycles between early endosomes and the trans-Golgi network and may play a role in exocytic vesicle formation. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq Oct 2011]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.