PrimePCR™ SYBR® Green Assay: G6PC2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0052819

List Price:    $146.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0045558
Assay Design:   exonic
Chromosome Location:   2:169757829-169757932question
Amplicon Length:   74
Splice Variants Targeted:   ENST00000574012 ENST00000572378 ENST00000573192 ENST00000375363 ENST00000429379 ENST00000421979

Gene Information

This gene encodes an enzyme belonging to the glucose-6-phosphatase catalytic subunit family. These enzymes are part of a multicomponent integral membrane system that catalyzes the hydrolysis of glucose-6-phosphate the terminal step in gluconeogenic and glycogenolytic pathways allowing the release of glucose into the bloodstream. The family member encoded by this gene is found in pancreatic islets and does not exhibit phosphohydrolase activity but it is a major target of cell-mediated autoimmunity in diabetes. Several alternatively spliced transcript variants of this gene have been described but their biological validity has not been determined. [provided by RefSeq Jul 2008]

Gene Symbol:   G6PC2
Gene Name:   glucose-6-phosphatase, catalytic, 2
Aliases:   IGRP, MGC141936
RefSeq:   NC_000002.11 NT_005403.17 NW_003315909.1 NG_011682.1
Ensembl:   ENSG00000152254
Entrez:   57818
UniGene:   Hs.283963
Chromosome Mapping:   2q24.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999100
y-intercept 35.790000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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