This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCEP0052754
PrimePCR™ PreAmp for SYBR® Green Assay: PIP4K2A, Human
PrimePCR™ Template for SYBR® Green Assay: PIP4K2A, Human
Phosphatidylinositol-54-bisphosphate the precursor to second messengers of the phosphoinositide signal transduction pathways is thought to be involved in the regulation of secretion cell proliferation differentiation and motility. The protein encoded by this gene is one of a family of enzymes capable of catalyzing the phosphorylation of phosphatidylinositol-5-phosphate on the fourth hydroxyl of the myo-inositol ring to form phosphatidylinositol-54-bisphosphate. The amino acid sequence of this enzyme does not show homology to other kinases but the recombinant protein does exhibit kinase activity. This gene is a member of the phosphatidylinositol-5-phosphate 4-kinase family. [provided by RefSeq Jul 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.