This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0052735
PrimePCR™ PreAmp for SYBR® Green Assay: MPP7, Human
PrimePCR™ Template for SYBR® Green Assay: MPP7, Human
Membrane-associated guanylate kinases (MAGUKs) are important adaptor proteins involved in the assembly of protein complexes at sites of cell-cell contact. They are found in synapses adherens junctions and tight junctions. All MAGUKs contain at least 1 PDZ domain an SH3 domain and a GUK domain and many contain 1 or 2 L27 domains which are involved in multimerization of MAGUKs. MPP7 belongs to the p55 stardust subfamily of MAGUKs which is named for a Drosophila gene required for establishment of cell polarity in the developing fly embryo (Bohl et al. 2007 [PubMed 17237226]).[supplied by OMIM Mar 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.